Crystal structure of the bacterial acetate transporter SatP reveals that it forms a hexameric channel.

ABSTRACT: Acetate is found ubiquitously in the natural environment and can be used as an exogenous carbon source by bacteria, fungi, and mammalian cells. A representative member of the acetate uptake transporter (AceTr) family named SatP (also yaaH) has been preliminarily identified as a succinate-acetate/proton symporter in Escherichia coli However, the molecular mechanism of acetate uptake by SatP still remains elusive. Here, we report the crystal structure of SatP from E. coli at 2.8 Å resolution, determined with a molecular replacement approach using a previously developed predicted model algorithm, which revealed a hexameric UreI-like channel structure. Structural analysis identified six transmembrane (TM) helices surrounding the central channel pore in each protomer and three con

SUBMITTER: Sun P 

PROVIDER: S-EPMC6302158 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature