Comprehensive analysis of N-glycans in IgG purified from ferrets with or without influenza A virus infection.

ABSTRACT: Influenza viruses cause contagious respiratory infections, resulting in significant economic burdens to communities. Production of influenza-specific Igs, specifically IgGs, is one of the major protective immune mechanisms against influenza viruses. In humans, N-glycosylation of IgGs plays a critical role in antigen binding and effector functions. The ferret is the most commonly used animal model for studying influenza pathogenesis, virus transmission, and vaccine development, but its IgG structure and functions remain largely undefined. Here we show that ferret IgGs are N-glycosylated and that their N-glycan structures are diverse. Using a comprehensive strategy based on MS and ultra-HPLC analyses in combination with exoglycosidase digestions, we assigned 42 N-

SUBMITTER: Zou G 

PROVIDER: S-EPMC6302183 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature