Analyzing the Dynamics of Single TBP-DNA-NC2 Complexes Using Hidden Markov Models.

ABSTRACT: Single-pair Förster resonance energy transfer (spFRET) has become an important tool for investigating conformational dynamics in biological systems. To extract dynamic information from the spFRET traces measured with total internal reflection fluorescence microscopy, we extended the hidden Markov model (HMM) approach. In our extended HMM analysis, we incorporated the photon-shot noise from camera-based systems into the HMM. Thus, the variance in Förster resonance energy transfer (FRET) efficiency of the various states, which is typically a fitted parameter, is explicitly included in the analysis estimated from the number of detected photons. It is also possible to include an additional broadening of the FRET state, which would then only reflect the inherent flexibility of the dynamic biolo

SUBMITTER: Zarrabi N 

PROVIDER: S-EPMC6302255 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature