NGmerge: merging paired-end reads via novel empirically-derived models of sequencing errors.

ABSTRACT:

Background

Advances in Illumina DNA sequencing technology have produced longer paired-end reads that increasingly have sequence overlaps. These reads can be merged into a single read that spans the full length of the original DNA fragment, allowing for error correction and accurate determination of read coverage. Extant merging programs utilize simplistic or unverified models for the selection of bases and quality scores for the overlapping region of merged reads.

Results

We first examined the baseline quality score - error rate relationship using sequence reads derived from PhiX. In contrast to numerous published reports, we found that the quality scores produced by Illumina were not substantially inflated above the theoretical values, once the reference genome was correcte

SUBMITTER: Gaspar JM 

PROVIDER: S-EPMC6302405 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature