Kinetics of the chromosome 14 microRNA cluster ortholog and its potential role during placental development in the pregnant mare.

ABSTRACT:

Background

The human chromosome 14 microRNA cluster (C14MC) is a conserved microRNA (miRNA) cluster across eutherian mammals, reported to play an important role in placental development. However, the expression kinetics and function of this cluster in the mammalian placenta are poorly understood. Here, we evaluated the expression kinetics of the equine C24MC, ortholog to the human C14MC, in the chorioallantoic membrane during the course of gestation.

Results

We demonstrated that C24MC-associated miRNAs presented a higher expression level during early stages of pregnancy, followed by a decline later in gestation. Evaluation of one member of C24MC (miR-409-3p) by in situ hybridization demonstrated that its cellular localization predominantly involved the chorion and allantoic

SUBMITTER: Dini P 

PROVIDER: S-EPMC6302407 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature