Improved protein production and codon optimization analyses in Escherichia coli by bicistronic design.

ABSTRACT: Different codon optimization algorithms are available that aim at improving protein production by optimizing translation elongation. In these algorithms, it is generally not considered how the altered protein coding sequence will affect the secondary structure of the corresponding RNA transcript, particularly not the effect on the 5'-UTR structure and related ribosome binding site availability. This is a serious drawback, because the influence of codon usage on mRNA secondary structures, especially near the start of a gene, may strongly influence translation initiation. In this study, we aim to reduce the effect of codon usage on translation initiation by applying a bicistronic design (BCD) element. Protein production of several codon-optimized gene variants is tested in parallel for a BCD

SUBMITTER: Nieuwkoop T 

PROVIDER: S-EPMC6302717 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature