Reverse transcription loop-mediated isothermal amplification to rapidly detect Rice ragged stunt virus.

ABSTRACT: Rice ragged stunt virus (RRSV) is a very important virus that infects rice and causes serious yield losses in Asian countries and other major rice planting areas. Thus, it is urgent to establish an efficient and practical approach for identification and diagnosis in the field. Our results indicated that reverse transcription loop-mediated isothermal amplification (RT-LAMP) reactions are more efficient and sensitive than RT-PCR for RRSV detection. The optimal LAMP conditions were as follows: 0.4-1.2 μM internal primers, 0.2-0.25 μM external primers, 0.8 μM loop primers, and incubation at 62 °C or 63 °C for 30 min. Furthermore, the RT-LAMP primers specifically targeted RRSV virus and resulted in typical waterfall-like bands by gel electrophoresis and sigmoidal amplification curves. Th

SUBMITTER: Lai D 

PROVIDER: S-EPMC6302892 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature