A high-throughput, whole cell assay to identify compounds active against carbapenem-resistant Klebsiella pneumoniae.

ABSTRACT: Enteric Gram-negative rods (GNR), which are frequent causes of community-acquired and nosocomial infections, are increasingly resistant to the antibiotics in our current armamentarium. One solution to this medical dilemma is the development of novel classes of antimicrobial compounds. Here we report the development of a robust, whole cell-based, high-throughput metabolic assay that detects compounds with activity against carbapenem-resistant Klebsiella pneumoniae. We have used this assay to screen approximately 8,000 fungal extracts and 50,000 synthetic compounds with the goal of identifying extracts and compounds active against a highly resistant strain of Klebsiella pneumoniae. The primary screen identified 43 active fungal extracts and 144 active synthetic compounds. Patulin, a known fu

SUBMITTER: Liao J 

PROVIDER: S-EPMC6303040 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature