Molecular characterization of flavonoid biosynthetic genes and accumulation of baicalin, baicalein, and wogonin in plant and hairy root of Scutellaria lateriflora.

ABSTRACT: Scutellaria lateriflora is well known for its medical applications because of the presence of flavanoids and alkaloids. The present study aimed to explore the molecular aspects and regulations of flavanoids. Five partial cDNAs encoding genes that are involved in the flavonoid biosynthetic pathway: phenylalanine ammonia lyase (SlPAL), cinnamate 4-hydroxylase (SlC4H), 4-coumaroyl CoA ligase (Sl4CL), chalcone synthase (SlCHS), and chalcone isomerase (SlCHI) were isolated from S. lateriflora. Organ expression analysis showed that these genes were expressed in all organs analyzed with the highest levels correlating with the richest accumulation of wogonin in the roots. Baicalin and baicalein differentially accumulated in S. lateriflora plants, with the highest concentration

SUBMITTER: Tuan PA 

PROVIDER: S-EPMC6303135 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature