Microsatellite primer development for the invasive perennial herb Gypsophila paniculata (Caryophyllaceae).

ABSTRACT:

Premise of the study

Gypsophila paniculata (baby's breath; Caryophyllaceae) is a herbaceous perennial that has invaded much of northern and western United States and Canada, outcompeting and crowding out native and endemic species. Microsatellite primers were developed to analyze the genetic structure of invasive populations.

Methods and results

We identified 16 polymorphic nuclear microsatellite loci for G. paniculata out of 73 loci that successfully amplified from a primer library created using Illumina sequencing technology. Microsatellite primers were developed to amplify di-, tri-, and tetranucleotide repeats and tested in three invasive populations in Michigan.

Conclusions

These markers will be useful in characterizing the genetic structure of inva

SUBMITTER: Leimbach-Maus HB 

PROVIDER: S-EPMC6303149 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature