Isolation and characterization of microsatellite loci from Pterocarya stenoptera (Juglandaceae).

ABSTRACT:

Premise of the study

Microsatellite markers of Pterocarya stenoptera (Juglandaceae) were developed for future studies on the population genetic diversity and spatial genetic structure of the species.

Methods and results

Based on Illumina sequencing of the transcriptome of P. stenoptera, a total of 2452 microsatellites were identified from 83,674 assembled unigenes. One hundred microsatellites were randomly selected to design amplification primer pairs. Of these, 15 were successfully amplified and displayed polymorphism. For these markers, the number of alleles per locus and population ranged from one to six. The levels of observed and expected heterozygosity varied from 0.000 to 1.000 and 0.000 to 0.718, respectively. Furthermore, all of the 15 loci were succes

SUBMITTER: Wang PF 

PROVIDER: S-EPMC6303151 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature