Microsatellite markers for the prized matsutake mushroom (Tricholoma matsutake, Tricholomataceae).

ABSTRACT:

Premise of the study

Novel and cost-effective microsatellite markers were developed to explore the population genetics, biogeographic structure, and evolutionary history of the prized Euro-Asian wild edible ectomycorrhizal fungus Tricholoma matsutake (Tricholomataceae).

Methods and results

Eighteen new polymorphic simple sequence repeat loci, detected from a microsatellite-enriched genomic library, were used to characterize 131 individuals from eight T. matsutake populations. The number of alleles ranged from two to 10, with averages of 1.42 to 3.22. Levels of observed and expected heterozygosity ranged from 0.00-1.00 and from 0.00-0.83, with mean values of 0.21 and 0.26, respectively. In total, 50% of the loci showed interspecific transferability and polymorph

SUBMITTER: Li JW 

PROVIDER: S-EPMC6303152 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature