Spatzle4 gene of silkworm, Bombyx mori: identification, immune response, and the effect of RNA interference on the antimicrobial peptides' expression in the integument.

ABSTRACT: Insects, including silkworms, can protect themselves from exotic invasions by innate immune responses. In the immune response of Drosophila, the activation of Toll receptors is strictly dependent on the product of Spatzle. In this study, we cloned the 1567 bp BmSpz4 cDNA which contained a complete 1386 bp open reading frame, encoding 461 amino acids, out of which the forgoing 19 residues were signal peptide. The result of the cDNA sequencing showed that we found a longer transcript than the one included in large scale full-Length cDNA sequencing data by Yoshitaka Suetsugu et al. in 2013. Several spliced variants of BmSpz4 have been found, based on our preliminary results. It was shown by the RT-PCR that BmSpz4 was expressed highest in the head, lower in the integument and testis. The expre

SUBMITTER: Huang X 

PROVIDER: S-EPMC6303178 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature