Ontology highlight
ABSTRACT:
SUBMITTER: Sanson KR
PROVIDER: S-EPMC6303322 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Nature communications 20181221 1
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene function. Here, we show that our recently-described CRISPRko library (Brunello) is more effective than previously published libraries at distinguishing essential and non-essential genes, providing approximately the same perturbation-level performance improvement over GeCKO libraries as GeCKO provided over RNAi. Additionally, we pr ...[more]