Ezh2 programs T_FH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf.

ABSTRACT: Ezh2 is an histone methyltransferase (HMT) that catalyzes H3K27me3 and functions in T_H1, T_H2, and Treg cells primarily via HMT activity. Here we show that Ezh2 ablation impairs T follicular helper (T_FH) cell differentiation and activation of the T_FH transcription program. In T_FH cells, most Ezh2-occupied genomic sites, including the Bcl6 promoter, are associated with H3K27ac rather than H3K27me3. Mechanistically, Ezh2 is recruited by Tcf1 to directly activate Bcl6 transcription, with this function requiring Ezh2 phosphorylation at Ser21. Meanwhile, Ezh2 deploys H3K27me3 to repress Cdkn2a expression in T_FH cells, where aberrantly upregulated p19Arf, a Cdkn2a protein product, triggers T_FH cell apoptosis and antagoni

SUBMITTER: Li F 

PROVIDER: S-EPMC6303346 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature