Flavin-based metabolic cycles are integral features of growth and division in single yeast cells.

ABSTRACT: The yeast metabolic cycle (YMC) is a fascinating example of biological organization, in which cells constrain the function of specific genetic, protein and metabolic networks to precise temporal windows as they grow and divide. However, understanding the intracellular origins of the YMC remains a challenging goal, as measuring the oxygen oscillations traditionally associated with it requires the use of synchronized cultures growing in nutrient-limited chemostat environments. To address these limitations, we used custom-built microfluidic devices and time-lapse fluorescence microscopy to search for metabolic cycling in the form of endogenous flavin fluorescence in unsynchronized single yeast cells. We uncovered robust and pervasive metabolic cycles that were synchronized with the cell divis

SUBMITTER: Baumgartner BL 

PROVIDER: S-EPMC6303410 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature