Three-Dimensional Optical Tweezers Tracking Resolves Random Sideward Steps of the Kinesin-8 Kip3.

ABSTRACT: The budding yeast kinesin-8 Kip3 is a highly processive motor protein that walks to the ends of cytoskeletal microtubules and shortens them in a collective manner. However, how exactly Kip3 reaches the microtubule end is unclear. Although rotations of microtubules in multimotored Kip3 gliding assays implied directed sideward switching between microtubule protofilaments, two-dimensional, single-molecule, optical-tweezers assays indicated that Kip3 randomly switched protofilaments. Here, we topographically suspended microtubules such that Kip3 motors could freely access the microtubules in three dimensions. Tracking single-motor-driven microspheres with a three-dimensional, zero-load, optical-tweezers-based force clamp showed that Kip3 switched protofilaments in discrete steps equally freque

SUBMITTER: Bugiel M 

PROVIDER: S-EPMC6303417 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature