Transmembrane Exchange of Fluorosugars: Characterization of Red Cell GLUT1 Kinetics Using ¹⁹F NMR.

ABSTRACT: We have developed a new approach, to our knowledge, to quantify the equilibrium exchange kinetics of carrier-mediated transmembrane transport of fluorinated substrates. The method is based on adapted kinetic theory that describes the concentration dependence of the transmembrane exchange rates of two competing, simultaneously transported species. Using the new approach, we quantified the kinetics of membrane transport of both anomers of three monofluorinated glucose analogs in human erythrocytes (red blood cells) using ¹⁹F NMR exchange spectroscopy. An inosine-based glucose-free medium was shown to promote survival and stable metabolism of red blood cells over the duration of the experiments (several hours). Earlier NMR studies only yielded the apparent rate constants and transm

SUBMITTER: Shishmarev D 

PROVIDER: S-EPMC6303418 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature