Automated sequence-level analysis of kinetics and thermodynamics for domain-level DNA strand-displacement systems.

ABSTRACT: As an engineering material, DNA is well suited for the construction of biochemical circuits and systems, because it is simple enough that its interactions can be rationally designed using Watson-Crick base pairing rules, yet the design space is remarkably rich. When designing DNA systems, this simplicity permits using functional sections of each strand, called domains, without considering particular nucleotide sequences. However, the actual sequences used may have interactions not predicted at the domain-level abstraction, and new rigorous analysis techniques are needed to determine the extent to which the chosen sequences conform to the system's domain-level description. We have developed a computational method for verifying sequence-level systems by identifying discrepancies between the

SUBMITTER: Berleant J 

PROVIDER: S-EPMC6303802 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature