Next-generation sequencing analysis reveals high bacterial diversity in wild venomous and non-venomous snakes from India.

ABSTRACT:

Background

The oral cavities of snakes are replete with various types of bacterial flora. Culture-dependent studies suggest that some of the bacterial species are responsible for secondary bacterial infection associated with snakebite. A complete profile of the ophidian oral bacterial community has been unreported until now. Therefore, in the present study, we determined the complete bacterial compositions in the oral cavity of some snakes from India.

Methods

Total DNA was isolated from oral swabs collected from three wild snake species (Indian Cobra, King Cobra and Indian Python). Next, the DNA was subjected to PCR amplification of microbial 16S rRNA gene using V3-region-specific primers. The amplicons were used for preparation of DNA libraries that were sequenced on an Ill

SUBMITTER: Krishnankutty SP 

PROVIDER: S-EPMC6303853 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature