Droplet digital PCR for the quantification of Alu methylation status in hematological malignancies.

ABSTRACT:

Background

Alu repeats, belonging to the Short Interspersed Repetitive Elements (SINEs) class, contain about 25% of CpG sites in the human genome. Alu sequences lie in gene-rich regions, so their methylation is an important transcriptional regulation mechanism. Aberrant Alu methylation has been associated with tumor aggressiveness, and also previously discussed in hematological malignancies, by applying different approaches. Moreover, today different techniques designed to measure global DNA methylation are focused on the methylation level of specific repeat elements. In this work we propose a new method of investigating Alu differential methylation, based on droplet digital PCR (ddPCR) technology.

Methods

Forty-six patients with hematological neoplasms were included in the

SUBMITTER: Orsini P 

PROVIDER: S-EPMC6303857 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature