Articular cartilage gene expression patterns in the tissue surrounding the impact site following applications of shear and axial loads.

ABSTRACT:

Background

Osteoarthritis is a degradative joint disease found in humans and commercial swine which can develop from a number of factors, including prior joint trauma. An impact injury model was developed to deliver in vitro loads to disease-free porcine patellae in a model of OA.

Methods

Axial impactions (2000 N normal) and shear impactions (500 N normal with induced shear forces) were delivered to 48 randomly assigned patellae. The patellae were then cultured for 0, 3, 7, or 14 days following the impact. Specimens in the tissue surrounding the loading site were harvested and expression of 18 OA related genes was studied via quantitative PCR. The selected genes were previously identified from published work and fell into four categories: cartilage matrix, degradative enzyme

SUBMITTER: McCulloch RS 

PROVIDER: S-EPMC6303924 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature