Fast quantitative urinary proteomic profiling workflow for biomarker discovery in kidney cancer.

ABSTRACT:

Background

Urine has evolved as a promising body fluids in clinical proteomics because it can be easily and noninvasively obtained and can reflect physiological and pathological status of the human body. Many efforts have been made to characterize more urinary proteins in recent years, but few have focused on the analysis throughput and detection reproducibility. Increasing the urine proteomic profiling throughput and reproducibility is urgently needed for discovering potential biomarker in large cohorts.

Methods

In this study, we developed a fast and robust workflow for streamlined urinary proteome analysis. The workflow integrate highly efficient sample preparation technique and urinary specific data-independent acquisition (DIA) approach. The performance of the workflow w

SUBMITTER: Lin L 

PROVIDER: S-EPMC6303996 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature