Magnetofection and isolation of DNA using polyethyleneimine functionalized magnetic iron oxide nanoparticles.

ABSTRACT: This study was carried out to develop a simple and efficient method to isolate DNA directly from biological samples using iron oxide nanoparticles (IONPs) functionalized with polyethyleneimine (PEI). IONPs were synthesized via co-precipitation method followed with direct attachment of branched PEI. Nanoparticles were characterized using STEM, FT-IR spectroscopy and XRD analysis. The binding capacity of synthesized PEI-IONPs for plasmid and genomic DNA was assessed using purified DNA samples. In order to elute bound DNA, elution conditions were optimized, changing pH, salt concentration and temperature. Synthesized PEI-IONPs were subjected to isolation of DNA from bacterial cell culture and from human blood. PCR and magnetofection of the enhanced green fluorescence protein (EGFP) were carri

SUBMITTER: Danthanarayana AN 

PROVIDER: S-EPMC6304157 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature