A newly developed tetraplex real-time RT-PCR for simultaneous screening of influenza virus types A, B, C and D.

ABSTRACT:

Background

Human- or avian-to-swine transmissions have founded several autonomously circulating influenza A virus (IAV) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (IBV) and C (ICV) in European swine, and of the recently detected novel animal influenza virus type D (IDV).

Objectives

Development of a cost-effective diagnostic tool for large-scale surveillance programmes targeting all four influenza virus types.

Methods

An influenza ABCD tetraplex real-time RT-PCR (RT-qPCR) was developed in the frame of this study. A selection of reference virus strains and more than 4000 porcine samples from a passive IAV surveillance programme in European swine with acute respiratory

SUBMITTER: Henritzi D 

PROVIDER: S-EPMC6304318 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature