Molecular Cytogenetics of Pisum sativum L. Grown under Spaceflight-Related Stress.

ABSTRACT: The ontogenesis and reproduction of plants cultivated aboard a spacecraft occur inside the unique closed ecological system wherein plants are subjected to serious abiotic stresses. For the first time, a comparative molecular cytogenetic analysis of Pisum sativum L. (Fabaceae) grown on board the RS ISS during the Expedition-14 and Expedition-16 and also plants of their succeeding (F1 and F2) generations cultivated on Earth was performed in order to reveal possible structural chromosome changes in the pea genome. The karyotypes of these plants were studied by multicolour fluorescence in situ hybridization (FISH) with five different repeated DNA sequences (45S rDNA, 5S rDNA, PisTR-B/1, microsatellite motifs (AG)₁₂, and (GAA)₉) as probes. A chromosome

SUBMITTER: Yurkevich OY 

PROVIDER: S-EPMC6304655 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature