Excitation-multiplexed multicolor superresolution imaging with fm-STORM and fm-DNA-PAINT.

ABSTRACT: Recent advancements in single-molecule-based superresolution microscopy have made it possible to visualize biological structures with unprecedented spatial resolution. Determining the spatial coorganization of these structures within cells under physiological and pathological conditions is an important biological goal. This goal has been stymied by the current limitations of carrying out superresolution microscopy in multiple colors. Here, we develop an approach for simultaneous multicolor superresolution imaging which relies solely on fluorophore excitation, rather than fluorescence emission properties. By modulating the intensity of the excitation lasers at different frequencies, we show that the color channel can be determined based on the fluorophore's response to the modulated excitat

SUBMITTER: Gomez-Garcia PA 

PROVIDER: S-EPMC6304964 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature