Homology modeling and in vivo functional characterization of the zinc permeation pathway in a heavy metal P-type ATPase.

ABSTRACT: The P1B ATPase heavy metal ATPase 4 (HMA4) is responsible for zinc and cadmium translocation from roots to shoots in Arabidopsis thaliana. It couples ATP hydrolysis to cytosolic domain movements, enabling metal transport across the membrane. The detailed mechanism of metal permeation by HMA4 through the membrane remains elusive. Here, homology modeling of the HMA4 transmembrane region was conducted based on the crystal structure of a ZntA bacterial homolog. The analysis highlighted amino acids forming a metal permeation pathway, whose importance was subsequently investigated functionally through mutagenesis and complementation experiments in plants. Although the zinc pathway displayed overall conservation among the two proteins, significant differences were observed, especially in the entr

SUBMITTER: Lekeux G 

PROVIDER: S-EPMC6305203 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature