Metabolic tracing reveals novel adaptations to skeletal muscle cell energy production pathways in response to NAD ⁺ depletion.

ABSTRACT: Background: Skeletal muscle is central to whole body metabolic homeostasis, with age and disease impairing its ability to function appropriately to maintain health. Inadequate NAD ⁺ availability is proposed to contribute to pathophysiology by impairing metabolic energy pathway use. Despite the importance of NAD ⁺ as a vital redox cofactor in energy production pathways being well-established, the wider impact of disrupted NAD ⁺ homeostasis on these pathways is unknown. Methods: We utilised skeletal muscle myotube models to induce NAD ⁺ depletion, repletion and excess and conducted metabolic tracing to provide comprehensive and detailed analysis of the consequences of altered NAD ⁺ metabolism on central carbon metabolic pat

SUBMITTER: Oakey LA 

PROVIDER: S-EPMC6305244 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature