Migratory Metrics of Wound Healing: A Quantification Approach for in vitro Scratch Assays.

ABSTRACT: Metastatic dissemination generates an aggressive disease facilitated by enhanced migratory and invasive properties. Experimental approaches employ several in vitro and in vivo assays toward quantification of these functionalities. In vitro assessments of cell motility often employ endpoint assays that rely on the global efficacy of wound closure and thwart quantification of migratory phenotypes observed during metastatic dissemination. Recent studies highlight the distinct signatures associated with individual vs. collective cell migration and necessitate the incorporation of these modalities into routine analyses. Advances in live cell imaging that permit real-time visualization of pathophysiological processes can be employed toward elucidating phenotypic plasticity a

SUBMITTER: Varankar SS 

PROVIDER: S-EPMC6305394 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature