Plasmodium falciparum var Gene Is Activated by Its Antisense Long Noncoding RNA.

ABSTRACT: Plasmodium falciparum erythrocyte membrane protein 1, encoded by var gene, is an immunodominant antigen mediating immune evasion in humans. At a given time, only a single var gene is commonly expressed in one parasite. However, the regulation mechanism of var transcription remains largely unknown. In this study, we identified the antisense long non-coding RNA (aslncRNA) derived from var intron as an activation factor for the corresponding var gene. The exogenous artificial var aslncRNA transcribed by T7 RNA polymerase from episome can specifically activate the homologous var gene, and the exogenous aslncRNA activates transcription of both var mRNA and endogenous aslncRNA in a manner independent of the conserved intron sequence

SUBMITTER: Jing Q 

PROVIDER: S-EPMC6305453 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature