Dynamics of the Hypoxia-Induced Tissue Edema in the Rat Barrel Cortex in vitro.

ABSTRACT: Cerebral edema is a major, life threatening complication of ischemic brain damage. Previous studies using brain slices have revealed that cellular swelling and a concomitant increase in tissue transparency starts within minutes of the onset of metabolic insult in association with collective anoxic spreading depolarization (aSD). However, the dynamics of tissue swelling in brain slices under ischemia-like conditions remain elusive. Here, we explored the dynamics of brain tissue swelling induced by oxygen-glucose deprivation (OGD) in submerged rat barrel cortex slices. Video monitoring of the vertical and horizontal position of fluorescent dye-filled neurons and contrast slice surface imaging revealed elevation of the slice surface and a horizontal displacement of the cortical tissue during

SUBMITTER: Juzekaeva E 

PROVIDER: S-EPMC6305551 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature