Development of a Rapid in planta BioID System as a Probe for Plasma Membrane-Associated Immunity Proteins.

ABSTRACT: Plant pathogens secrete effector molecules that suppress the plant immune response to facilitate disease development. AvrPto is a well-studied effector from the phytopathogenic bacterium Pseudomonas syringae. Here we utilize an in planta proximity dependent biotin ligase labeling technique (BioID) in combination with AvrPto to identify proximal proteins that are potential immune system components. The labeling technique biotinylated proteins proximal to AvrPto at the plasma-membrane allowing their isolation and identification by mass spectrometry. Five AvrPto proximal plant proteins (APPs) were identified and their effect on plant immune function and growth was examined in Nicotiana benthamiana leaves. One protein identified, RIN4, is a central immune component previou

SUBMITTER: Conlan B 

PROVIDER: S-EPMC6305590 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature