MicroRNA-451a acts as tumor suppressor in cutaneous basal cell carcinoma.

ABSTRACT:

Background

Basal cell carcinoma (BCC) is the most common type of skin cancer. The underlying mechanism leading to BCC formation is not fully uncovered. The aim of this study was to characterize miRNA-451a as a novel tumor suppressor in cutaneous BCC.

Methods

We first evaluated miRNA-451a level in human BCC clinical tissues and inducible BCC mouse model. Then we studied the impact of overexpressing or inhibiting miR-451a in cell proliferation, colony formation potential, and cell cycle pattern. Next, we employed luciferase reporter assay and western blotting to evaluate TBX1 as a downstream target of miRNA-451a. Lastly, we confirmed TBX1 expressional change in BCC tissues by qPCR.

Results

miRNA-451a was significantly reduced in human BCC tissues. The downregulation of

SUBMITTER: Sun H 

PROVIDER: S-EPMC6305666 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature