Particulate matter induces inflammatory cytokine production via activation of NFκB by TLR5-NOX4-ROS signaling in human skin keratinocyte and mouse skin.

ABSTRACT: Particulate matter (PM) increases levels of pro-inflammatory cytokines, but its effects on the skin remain largely unknown. We investigated the signal transduction pathway and epigenetic regulatory mechanisms underlying cellular inflammation induced by PM with a diameter of ≤ 2.5 (PM_2.5) in vitro and in vivo. PM_2.5-treated skin keratinocytes produced various inflammatory cytokines, including IL-6. The binding of PM_2.5 to TLR5 initiated intracellular signaling through MyD88, and led to the translocation of NFκB to the nucleus, where it bound the NFκB site within IL-6 promoter. Furthermore, PM_2.5 induced a direct interaction between TLR5 and NOX4, and in turn induced the production of ROS and activated NFκB-IL-6 downstream, which was prevented by s

SUBMITTER: Ryu YS 

PROVIDER: S-EPMC6305701 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature