MicroRNA-562 negatively regulated c-MET/AKT pathway in the growth of glioblastoma cells.

ABSTRACT:

Background

MicroRNA-562 (miR-562) has been found to possess anti-cancer function in certain tumors. However, the function of miR-562 in glioblastoma (GBM) is still not fully understood.

Purpose

The aim at present study is to analyze the function of miR-562 and its possible target in GBM cells.

Patients and methods

In the present study, a total of 80 GBM samples and 16 adjacent noncancerous tissues were used to examine the expression of miR-562 and c-MET. In order to gain a deep insight into the molecular network of miR-562 and c-MET in GBM, the miR-562 mimic and inhibitor were transfected into two GBM cell lines (U251 and U87), respectively. Meanwhile, lentiviral vector was used to mediate overexpression of c-MET. Cell proliferation was examined via Cell Counting Kit-

SUBMITTER: Nie X 

PROVIDER: S-EPMC6306063 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature