Endemic dissemination of different sequence types of carbapenem-resistant Klebsiella pneumoniae strains harboring bla _NDM and 16S rRNA methylase genes in Kerman hospitals, Iran, from 2015 to 2017.

ABSTRACT:

Introduction

The emergence and spread of Klebsiella pneumoniae strains resistant to multiple antimicrobial agents are considered as a serious challenge for nosocomial infections.

Materials and methods

In this study, 175 nonrepetitive clinical isolates of K. pneumoniae were collected from hospitalized patients in Kerman, Iran. Extended-spectrum β-lactamases (ESBLs), AmpC, and carbapenemase-producing isolates were recognized by phenotypic methods. The resistance genes including efflux pumps oqxA/oqxB, 16S rRNA methylase, ESBL, AmpC, and carbapenemase were detected by PCR-sequencing method. Molecular typing was performed by enterobacterial repetitive intergenic consensus-PCR and multilocus sequence typing methods among bla _NDM-posit

SUBMITTER: Kiaei S 

PROVIDER: S-EPMC6306073 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature