Development of Onchocerca volvulus in humanized NSG mice and detection of parasite biomarkers in urine and serum.

ABSTRACT:

Background

The study of Onchocerca volvulus has been limited by its host range, with only humans and non-human primates shown to be susceptible to the full life cycle infection. Small animal models that support the development of adult parasites have not been identified.

Methodology/principal findings

We hypothesized that highly immunodeficient NSG mice would support the survival and maturation of O. volvulus and alteration of the host microenvironment through the addition of various human cells and tissues would further enhance the level of parasite maturation. NSG mice were humanized with: (1) umbilical cord derived CD34+ stem cells, (2) fetal derived liver, thymus and CD34+ stem cells or (3) primary human skeletal muscle cells. NSG and humanized NSG mice were infected wit

SUBMITTER: Patton JB 

PROVIDER: S-EPMC6306240 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature