SRSF1 modulates PTPMT1 alternative splicing to regulate lung cancer cell radioresistance.

ABSTRACT:

Background

Radioresistance is the major cause of cancer treatment failure. Additionally, splicing dysregulation plays critical roles in tumorigenesis. However, the involvement of alternative splicing in resistance of cancer cells to radiotherapy remains elusive. We sought to investigate the key role of the splicing factor SRSF1 in the radioresistance in lung cancer.

Methods

Lung cancer cell lines, xenograft mice models, and RNA-seq were employed to study the detailed mechanisms of SRSF1 in lung cancer radioresistance. Clinical tumor tissues and TCGA dataset were utilized to determine the expression levels of distinct SRSF1-regulated splicing isoforms. KM-plotter was applied to analyze the survival of cancer patients with various levels of SRSF1-regulated splicing isoforms.

SUBMITTER: Sheng J 

PROVIDER: S-EPMC6306353 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature