An ameloblastin C-terminus variant is present in human adipose tissue.

ABSTRACT:

Objective

Transcriptional regulatory elements in the ameloblastin (AMBN) promoter indicate that adipogenesis may influence its expression. The objective here was to investigate if AMBN is expressed in adipose tissue, and have a role during differentiation of adipocytes.

Design

AMBN expression was examined in adipose tissue and adipocytes by real-time PCR and ELISA. Distribution of ameloblastin was investigated by immunofluorescence in sections of human subcutaneous adipose tissue. The effect of recombinant proteins resembling AMBN and its processed products on proliferation of primary human pre-adipocytes and murine 3T3-L1 cell lines was measured by [³H]-thymidine incorporation. The effect on adipocyte differentiation was evaluated by the expression profile

SUBMITTER: Stakkestad O 

PROVIDER: S-EPMC6307104 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature