Analytical validation of a prognostic prostate cancer gene expression assay using formalin fixed paraffin embedded tissue.

ABSTRACT:

Background

There is a clear need for assays that can predict the risk of metastatic prostate cancer following curative procedures. Importantly these assays must be analytically robust in order to provide quality data for important clinical decisions. DNA microarray based gene expression assays measure several analytes simultaneously and can present specific challenges to analytical validation. This study describes the analytical validation of one such assay designed to predict metastatic recurrence in prostate cancer using primary formalin fixed paraffin embedded tumour material.

Methods

Accuracy was evaluated with a method comparison study between the assay development platform (Prostate Disease Specific Array) and an alternative platform (Xcel™ microarray) using 50 formali

SUBMITTER: Medlow PW 

PROVIDER: S-EPMC6307209 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature