Mapping of the complement C1q binding site on Trichinella spiralis paramyosin.

ABSTRACT:

Background

Trichinella spiralis is a tissue-dwelling parasite has developed the ability to evade the host immune attack to establish parasitism in a host. One of the strategies evolved by the nematode is to produce proteins that immunomodulate the host immune system. TsPmy is a paramyosin secreted by T. spiralis on the surface of larvae and adult worms that can interact with complement components C1q and C8/C9 to compromise their activation and functions. To better understand the mechanism of TsPmy involved in the C1q inactivation and immune evasion, the C1q-binding site on TsPmy was investigated.

Methods

The TsPmy C1q-binding site was investigated by sequential narrow-down fragment expression in bacteria and peptide binding screening. C1q binding activity was identified by

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6307294 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature