Ischemia-reperfusion injury in a rat microvascular skin free flap model: A histological, genetic, and blood flow study.

ABSTRACT: Ischemia reperfusion injury is associated with tissue damage and inflammation, and is one of the main factors causing flap failure in reconstructive microsurgery. Although ischemia-reperfusion (I/R) injury is a well-studied aspect of flap survival, its biological mechanisms remain to be elucidated. To better understand the biological processes of ischemia reperfusion injury, and to develop further therapeutic strategies, the main objective of this study was to identify the gene expression pattern and histological changes in an I/R injury animal model. Fourteen rats (n = 7/group) were randomly divided into control or ischemia-reperfusion group (8 hours of ischemia). Microsurgical anastomoses were objectively assessed using transit-time-ultrasound technology. Seven days after surgery, flap s

SUBMITTER: Ballestin A 

PROVIDER: S-EPMC6307726 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature