Efficient method for isolation of reticulocyte RNA from healthy individuals and hemolytic anaemia patients.

ABSTRACT: Despite enormous progress and development of high-throughput methods in genome-wide mRNA analyses, data on the erythroid transcriptome are still limited, even though they could be useful in medical diagnostics and personalized therapy as well as in research on normal and pathological erythroid maturation. Although obtaining normal and pathological reticulocyte transcriptome profiles should contribute greatly to our understanding of the molecular bases of terminal erythroid differentiation as well as the mechanisms of the hematological diseases, a basic limitation of these studies is the difficulty of efficient reticulocyte RNA isolation from human peripheral blood. The restricted number of possible parallel experiments primarily concern healthy individuals with the lowest number of reticul

SUBMITTER: Skulski M 

PROVIDER: S-EPMC6307756 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature