Spec-seq unveils transcriptional subpopulations of antibody-secreting cells following influenza vaccination.

ABSTRACT: Vaccines are among the most effective public health tools for combating certain infectious diseases such as influenza. The role of the humoral immune system in vaccine-induced protection is widely appreciated; however, our understanding of how antibody specificities relate to B cell function remains limited due to the complexity of polyclonal antibody responses. To address this, we developed the Spec-seq framework, which allows for simultaneous monoclonal antibody (mAb) characterization and transcriptional profiling from the same single cell. Here, we present the first application of the Spec-seq framework, which we applied to human plasmablasts after influenza vaccination in order to characterize transcriptional differences governed by B cell receptor (BCR) isotype and vaccine reactivity.

SUBMITTER: Neu KE 

PROVIDER: S-EPMC6307935 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature