Naive CD8⁺ T-Cells Engage a Versatile Metabolic Program Upon Activation in Humans and Differ Energetically From Memory CD8⁺ T-Cells.

ABSTRACT: Background: Characterization of the intracellular biochemical processes that regulate the generation and maintenance of effector and memory CD8⁺ T-cells from naïve precursors is essential for our understanding of adaptive immune responses and the development of immunotherapies. However, the metabolic determinants of antigen-driven activation and differentiation remain poorly defined, especially in humans. Methods: We used a variety of different approaches, including gene expression profiling and measurements of nutrient flux, to characterize the basal and activation-induced energetic requirements of naïve and phenotypically-defined subsets of human memory CD8⁺ T-cells. Findings: Profound metabolic differences were apparent as a function of differe

SUBMITTER: Nicoli F 

PROVIDER: S-EPMC6308131 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature