Initial Assessment of Variability of Responses to Toxicants in Donor-Specific Endothelial Colony Forming Cells.

ABSTRACT: There is increased interest in using high throughput in vitro assays to characterize human population variability in response to toxicants and drugs. Utilizing primary human endothelial colony-forming cells (ECFCs) isolated from blood would be highly useful for this purpose because these cells are involved in neonatal and adult vasculogenesis. We characterized the cytotoxicity of four known toxic chemicals (NaAsO₂, CdCl₂, tributyltin [TBT], and menadione) and their four relatively nontoxic counterparts (Na₂HAsO₄, ZnCl₂, SnCl₂, and phytonadione, respectively) in eight ECFC clones representing four neonatal donors (2 male and 2 female donors, 2 clones per donor). ECFCs were exposed to 9 concentrations of each chemical i

SUBMITTER: Filonov D 

PROVIDER: S-EPMC6308159 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature