Quantifying site-specific chromatin mechanics and DNA damage response.

ABSTRACT: DNA double-strand breaks pose a direct threat to genomic stability. Studies of DNA damage and chromatin dynamics have yielded opposing results that support either increased or decreased chromatin motion after damage. In this study, we independently measure the dynamics of transcriptionally active or repressed chromatin regions using particle tracking microrheology. We find that the baseline motion of transcriptionally repressed regions of chromatin are significantly less mobile than transcriptionally active chromatin, which is statistically similar to the bulk motion of chromatin within the nucleus. Site specific DNA damage using KillerRed tags induced in loci within repressed chromatin causes an increased motion, while loci within transcriptionally active regions remains unchanged at simi

SUBMITTER: Whitefield DB 

PROVIDER: S-EPMC6308236 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature