Molecular Assessment of C4d-Positive Renal Transplant Biopsies Without Evidence of Rejection.

ABSTRACT:

Introduction

Immunohistochemical staining for C4d in peritubular capillaries has been part of antibody-mediated rejection (AbMR) definition in the Banff Classification for Allograft Pathology since 2003. However, it has limited sensitivity and specificity, therefore the clinical significance of C4d-positive biopsies without evidence of rejection (C4d+ WER) is unknown. We investigated the transcript levels of genes associated with AbMR in C4d+ WER biopsies from both ABO-compatible and incompatible renal transplant patients.

Methods

RNA was extracted from formalin-fixed paraffin-embedded renal transplant biopsies (n = 125) and gene expression analysis of 35 AbMR-associated transcripts carried out using the NanoString nCounter system.

Results

AbMR-associated trans

SUBMITTER: Dominy KM 

PROVIDER: S-EPMC6308373 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature