Evaluation of top-down mass spectral identification with homologous protein sequences.

ABSTRACT:

Background

Top-down mass spectrometry has unique advantages in identifying proteoforms with multiple post-translational modifications and/or unknown alterations. Most software tools in this area search top-down mass spectra against a protein sequence database for proteoform identification. When the species studied in a mass spectrometry experiment lacks its proteome sequence database, a homologous protein sequence database can be used for proteoform identification. The accuracy of homologous protein sequences affects the sensitivity of proteoform identification and the accuracy of mass shift localization.

Results

We tested TopPIC, a commonly used software tool for top-down mass spectral identification, on a top-down mass spectrometry data set of Escherichia coli K12 MG1655,

SUBMITTER: Li Z 

PROVIDER: S-EPMC6309053 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature